The purpose of the experiment is to test the effect of increased temperature on the permeability of beetroot cell membran Essay Example

The purpose of the experiment is to test the effect of increased temperature on the permeability of beetroot cell membran Essay Background information- The cell membrane surrounds all living things and is partially permeable so as to serve as a boundary between cell and environment and control substances that are allowed in and out of the cell. Cell membranes are made up of phospholipids, carbohydrates and proteins.The phospholipids are arranged in a way that their polar hydrophilic (water attracting) phosphate heads face outwards and their non-polar (hydrophobic) fatty acid tails face inwards. The phospholipids are arranged in a bi-layer. The hydrophobic layers act as a barrier to some molecules.There are also other molecules which are indented into the phospholipids such as Proteins. Proteins are tertiary structures made up of coiled and folded strings of amino acids which are very strong and held in place by peptide bonds. Proteins are responsible for most of the cells properties and some proteins are involved in transporting substances across the membrane while others are involved in maintaining the cells shape. However at very high temperatures the binds holding the . break and thus the proteins lose their structure and stability. This increases the pigmentation released.In the cells of a beetroot plant, a substance called betalins is found in the membrane vacuoles, which gives beetroot its red colour. Normally the pigments cannot pass through the membranes but at higher temperatures the cell becomes damaged and the betalins will bleed from the cells. I am going to investigate the pigment lost at different temperatures.Hypothesis- an increase in temperature will lead to more damage to the cell membranes, which will increase their permeability, and therefore allow more of the pigment to be released.Expected result- the following temperatures will be used to measure the absorbency, 0C, 10C, 20C, 30C, 40C, 50C, 60C, 70C. as using any higher or lower may take up to much time in getting to the required temperature. expect the graph for the results to look as follows::increasing the temp erature will cause the partially permeable membrane of the Beetroot to become damaged and so it will be less rigid. Also I predict that after 40C the proteins in the cell membrane will start to denature as they will reach the optimum temperature. This will increase the permeability allowing more colour to be released. I predict that when I test a small amount of the water which contained the beetroot in the colorimeter, the higher the temperature of the water the higher the reading will be on the colorimeter as less light will pass through.Apparatus- The following apparatus will be needed in the investigation;Raw beetroot Preferably the same type and size of beetroot will be needed to make the investigation fair and more reliableSize 4 cork borer to obtain the beetroot pieces as it will enable me to have pieces and radius of the same size and ensure that the dependant variables are kept the same.White tile cut down on to this with the knife so that I do not damage the desk or risk h urting my handKnife to measure cut the beetroot into 1cm length slices.Ruler to measure and the beetroot into 1cm length slices.Water baths I will also need a bunsen burner to heat the water. I feel a bunsen burner is a better option to use than a kettle as I can get it to the required temperature more easily and also because the kettle would have more impurities, whereas I can wash the beaker before hand.Plastic beaker. A beaker will then be needed to place the water when it is being heated. I will use a 250ml beaker as I can get all the water in instead of having to add more each time.2 boiling tube racks2 test tube racks which I can place the tubes in.Crushed ice I will need ice for temperatures below the room temperature.Boiling tubes I will then need to get 8 test tubes and instead of small beaker as I can place the tubes in a rack so there are more stable and also so that less space is taken up in the desk as the beaker is widerThermometer I will also have to use a 0-90 thermo meter to take readings of the temperature. I will use a 0-90 C thermometer as the temperatures I need are below this and so there is no point using a larger one. Also it will be easier to read with a smaller scale. Ice will also be needed to get the temperatures lower than room temperatureColorimeter Colorimeters are very useful tools for obtaining quantitative data by following reactions that involve a change in colour or opacity. They take the guesswork out of matching colours or end points in experiments. Attaching the colorimeter to a datalogger allows you to see the progress of the reaction, and to create a permanent record of the whole experiment. à ¯Ã‚ ¿Ã‚ ½Datalogging is an extension of normal scientific enquiry techniques.1 Datalogging improves the validity of the data2 Datalogging removes the need for long periods of repetitive data-recording Transmission is not a linear scale, and is normally used when you are:à ¯Ã‚ ¿Ã‚ ½following a trend in the reactionà ¯Ã‚ ¿Ã‚ ½foll owing a reaction where the concentrations of the products creating the colourchange are unknown.As the transmission scale is not linear, it is not directly related to the concentration of thechemicals in a solution that create a colour change.Absorbance is a linear scale. It can be used when you are:à ¯Ã‚ ¿Ã‚ ½calibrating the colour change to a known concentration value, for example finding theconcentration of sugar in a sample.Cuvettes I will use a curvette to put the solutions in; I will place 2cm in each curvette using a 2cm pipette so that each has the same volume and so it is far more reliable to compare the results. When using the colour meter I will measure the absorbency of the distilled water and then compare this to the different beetroot concentrations. I will then need to measure the solutions against a colorimeter I will use a colorimeter rather than a chart as the sensor is sensitive to light and is in the same position all the time which will give far more accurate m easurements of absorbency than interpreting using a scale. Cuvettes are designed to be optically identical to each other. Many have a small mark on them so you can make sure the same faces are always lined up with the light source and sensor. If there is no markalready present, you can add a small mark at the very top of the cuvette, where it doesnot interfere with the passage of light through the test solution.Stopclock A stopwatch will also be required to measure the time for each experiment, so that I get a fair and more precise time for each, instead of relying on a watch or clock.Distilled water I will use distilled Water, as this will ensure a more accurate result as normal tap water has chemicals added which can affect the experiment.Pipette For transferring the water in to a measuring cylinder a 5cm pipette will be used as the beaker could be very hot to hold and transfer the water. Also I can get more accurate measurements with the pipette. I will transfer the water into a 50ml measuring cylinder as I may not get exactly 5cm each time in the pipette and so measuring it before would be more accurate.Small measuring cylindersforceps whilst transferring the beetroot in to the water so the beetroot does not come into contact with the skin and so that I do not involuntarily affect the permeability of the cell membrane.500ml beaker so that the beaker with the distilled water can be placed in this.tripod and a gauge to place the beaker on whilst it is getting heated, so direct heat does not come in contact with the glass, as it could get very hot.heatproof mat will need to be placed under all this to keep the Bunsen burner on something safe and also so that it remains stable and there is less risk of it falling over.goggles and a laboratory coat at all times to ensure safety and reduce the risk of any accidents. Also to reduce staining as the beetroot juiceMethod- start by wearing a laboratory coat and goggles at all times to ensure safety to the eye body. t hen wash my hands so that if they have any substances they do not affect my experiment by coming into contact with the beetroot or water. then obtain all the equipment needed for the investigation so that the procedure is orderly and I do not have to look for things in between the experiment.using a cork borer and pushing down onto a white tile cut out 8 cylindrical discs of beetroot. Next I will measure 1cm length of beetroots and cut out 8 pieces using the knife and cutting down onto a white tile. I will then get 8 test tubes for all the different temperatures and wash and dry the tubes so that no substance remains as this could affect the results. Then I will place the tubes in a test tube rack so they are on a flat surface and so it is easy to transfer the right amount of liquid. Using a size 4 cork borer and a knife, ruler and white tile to aide me I will cut 8 identical pieces of beetroot which are 1cm in length. I will use the same diameter corer and cut all the beetroots to the same length so that the surface area of each beetroot is similar and that the dependant variable is kept the same. I will then using tongs to place the beetroot pieces in a beaker of distilled water and leave overnight so that excess dye is washed away. Because the beetroot has been cut part of the cell membranes will be broken and therefore the excess dyewill leak out. By leaving them overnight it will ensure that the results are reliable.I will get the normal distilled water which will be at room temperature at about 34C and place it in a beaker. I will then add ice to a larger beaker and place the water beaker in this. I will work by starting with the 30C and then working down to 0C, adding more ice if needed. I have decided to carry out the investigation this way so that I can get the required temperature the most quickly (from room temp) so I do not have to wait too long. For the temperatures above room temperature i.e. 40-70C I will start with 70C degrees and work my way d own. This is so that after I have heated the water, I will not have to heat it again as it will cool so I will have the required temperature. This way I can make full use of the time.I will then get the 5cm pipette and use it to transfer water into a measuring cylinder. I will measure 20cm of water I have decide to use 20cm because I feel that it is enough to cover the beetroot and a suitable volume to measure for just 20 minutes.Next I will get forceps to transfer the beetroot into the test tube and start timing as soon as it is dropped in. I will time for 20 minutes to allow time to carry out each experiment in the time available and also that I carry the experiment out for a reliable amount of time. I feel that any time less than 20 minutes would not be sufficient. I will then stop the experiment after 20 minutes and use a 2cm pipette and place the solution in to a curvette to measure the absorbency. I will first measure the absorbency of distilled water by placing it into the sl ot. Test the logger before use with a simple sensor (e.g. temperature) that does notrequire any special set-up procedure. This can confirm that the equipment is working,and helps you check that you understand how to use the equipment before you collect your real data.I expect the reading to be 0. Then I will press r on the colorimeter and place the solution in to compare it. I will repeat this experimental procedure for all the temerparures below room temperature adding more ice when required.For temperatures above the room temperature i.e. 40C, 50C, 60C, 70C I will need to heat the distilled water. I will heat the water above 70C work my way down the measurements. I have decided to this as I can the next temperature as it is cooling from the previous one instead of re-heating. Thus I will place the beaker on the tripod, making sure that there is gauze below it and a heatproof mat under all this. When lighting the bunsen burner with a matchstick I will make sure that the hole is on so that the flame is visible, thus reduce the risk of an accident. I will use a thermometer to measure the temperature and once the solution is heated I will once again use a pipette to transfer the water in to a measuring cylinder. I will start transferring the water slightly above the required temperature so that by the time I have transferred it all, it will have fell to the required temperature. After measuring 20cmand checking that the water has fallen to the required temperature I will transfer it into the test tube. I will again use forceps to drop the beetroot into water and start timing as soon as the beetroot is in. I will measure the absorbency as before. I will then reduce the heat of the burner and measure each of the remaining temperatures following the same method. I will then wash my hands at the end of the experiment just to make sure that if any particles got to my hand they are removed.I am going to use a 10C space for the temperature as I think that using five wo uld be too small to distinguish trends, and more than that would also prove to be difficult.Before I test the samples of water I will first test the distilled water and use it as a control to see the difference in water colour. Using a pipette I will transfer 2cm of water from the tubes into the cuvettes. I will set the colorimeter to 0% which will give a blue/green colour. I will then take a reading of the colour absorbency in the order that I started. I will keep the colorimeter on 0% absorbance and repeat for the rest of the temperatures.I will draw up a graph of the results so that I can clearly distinguish the trends and that accurate conclusions can be made.Plotting a calibration curveTo relate readings of absorbance to actual concentrations, you need to make acalibration curve. Make up a series of solutions of known concentration, and takereadings of the absorbance values of these solutions. Plot absorbance values against the concentrations of these standard solutions. You ca n then use this calibration curve to convert absorbance values from your experiment to concentrations. If you are doing a rate experiment, then the absorbance is directly related to concentration, so a graph showing the change in absorbance over time is a genuine rate graph.Fair testing- To ensure that the test is fair so reliable and accurate results are obtained I will make sure the following is done:I have considered how to keep factors that affect the rate of movement across membrane constant and only change the independent variable. The independent variable in my investigation will be the temperature, as this will be constantly getting higher each time. Thus the dependant variable will be the pigmentation released. All the other variables will be kept constant as otherwise it could affect the results. For example the type of the beetroot should be the same; if not then this could affect what temperatures the membrane denatures because of the different prior environments of the beetroot.I will make sure that I wipe the test tubes after rinsing them so any excess water is removed.I must also ensure that the same volume of water is placed in each of the tubes as a higher volume in one tube could affect the diffusion. Thus I will measure the required volume after heating the water. This is because if I measured it first then a lot would evaporate with the heat and the investigation would not be fair.I will try to move the test tubes as least as possible in between the experiments so I do not speed up any reaction involuntary. Furthermore I will work on a flat surface, so as to make sure there is the right amount of volume and when transferring the water from the pipette to the test tube, I will try not to spill any.I will use the same beetroot for the whole experiment as using a different beetroot may affect the results. This is because a particular beetroot may contain more betalins which could result in a faster rate at which the dye is released. This would not make the experiment a fair test.ame sized cork borer (number 4) to ensure that all the beetroot pieces are the same width in diameter. A larger surface area will cause faster diffusion as more of the membrane will be exposed. (Ficks law)volume of water in each experiment must be consistent as more or less water could affect the rate of movement across the cell membrane.time the beetroot are left in the water. If they were all kept to different times then the results would not be reliable enough to draw up a conclusion and say if the results agree with the hypothesis.The independent variable will be the temperature of the water the beetroot pieces will be placed in. These temperatures will vary from 7à ¯Ã‚ ¿Ã‚ ½C to 73c.I will make sure before using the boiling tubes that they are clean and if not they are properly cleaned and dried so as not to contaminate the water with anything that is left behind in the tubes. This is because if the water got dirty this could possibly affec t the results.I will make sure than once the beetroots are in the boiling tubes I do not keep taking them out again or that I do not shake the tubes but leave them in the racks. This is because by extracting the beetroot or shaking the tubes it may speed up or slow dont the amount of dye that is released.Finally when using the calorimeter I must make sure that each sample is compared against the distilled water, and not another sample.Safety- Safety is very important in any scientific experiment. Before I start the experiment I will wear goggles and a laboratory coat so any risk of accidents is reduced. Also I will tie back any lose clothing and hair as they could come in the way of the experiment and cause an accident. I must make sure that I do not eat/drink/chew during the experiment as the products could be contaminated. Also I will wash my hands before and after the experiment so any substances are removed, and do not affect the investigation or cause harm to me.Whilst using th e cork borer and knife I will make sure that I always cut down onto a tile so that I reduce the risk of an accident to my hand.When heating the eater with the Bunsen burner I will have to be very careful with the fire and make sure that nothing is in the way. Also I will keep the flame visible so that I can see and be aware of the flame. After boiling the water in a kettle I will make sure that I pick the kettle up by the handle and I do not touch the outercase as it will be very hot.I will take extra caution whilst handling glassware as it could easily be smashed. Because the beaker could be very hot at times I will use a pipette to transfer the liquid so that I do not have to lift the beaker.I will make sure that the desktop I am working on is clean and has no obstructions, all stools, books and belongings and unwanted equipments I will put away as they could cause accidents. I will work on a flat surface to minimise the occurrence of an accident, if there are any spillages/ accid ents I will clean/report them immediately.I know how to use equipEthical issues- In this experiment an edible ingredient is going to be used. However I must make sure that because it is being used for the investigation that it is not consumed prior or after the investigation. Consuming it after the experiment could cause harm to the body.Results- I have recorded the results in a table as it is more systematic and so easier for me to interpret and distinguish any trends. Also it will be easier for me to identify any errors or anomalies. What makes a good table?A good table of results should have:Temperature C% Absorbency0C0.0010C0.0020C0.1030C0.1740C0.1950C0.2360C0.3070C0.34à ¯Ã‚ ¿Ã‚ ½an informative titleà ¯Ã‚ ¿Ã‚ ½units in headings, not next to the numerical data.A graph is a visual representation of data. Numerical data should be presented in a way that helps in the identification of any trends or patterns in the data.more easily seen in a graph than in a table, and anomalies mor e easily identified.Line graphsA line graph can be used to show relationships in data which are not immediately obvious from tables (Figure 4).When drawing any type of graph with axes, remember always to include:à ¯Ã‚ ¿Ã‚ ½an informative titleà ¯Ã‚ ¿Ã‚ ½sensible scales on each axis, if appropriateà ¯Ã‚ ¿Ã‚ ½labels on both axesà ¯Ã‚ ¿Ã‚ ½units on both axes, if appropriateà ¯Ã‚ ¿Ã‚ ½a key.The general trend of the results is obvious, as the temperature is increased the beetroot let out more color, thus the water was darker. This backs up my hypothesis in which I stated that the more heat added, the more dye will be released as more damage is done to membranes within the cell. And so the permeability of the cell membrane is directly proportional to the temperature. This is because as temperature rises the movement of the phospholipids increases allowing more of the dye to move across the membrane.I can identify that the results overall follow a pattern and that as the temperature i ncreases so does the absorbency. The biggest difference in absorbency was between 20 and 30C from 0.10 to 0.17 and 50C to 60C, from 0.23 to 0.30.From 0à ¯Ã‚ ¿Ã‚ ½C to 20à ¯Ã‚ ¿Ã‚ ½C, theres a slight increase in the average percentage of absorbeny. This is because when water freezes, it expands and this makes the cell membrane burst. The bursting results in more pigments leaking.In general between 20à ¯Ã‚ ¿Ã‚ ½C and 60à ¯Ã‚ ¿Ã‚ ½C, as the temerature increases more pigments are leaking out thus the darker the colour. This is because the cell becomes less permeable.Spearmans rank correlationA Spearmans rank correlation test will tell you whether two variables are correlated, i.e. whether a change in one variable is accompanied by a change in the other variable. It will tell you whether the relationship is a positive correlation (both go up together) or a negative correlation (one goes up as the other goes down) and the strength of any correlation.For any Spearmans rank correlation you do, the null hypothesis will be:There is no correlation between the two variables.You can use this test on interval or ordinal data and the data will always be in matched pairs. This means that one piece of data is associated with one other piece of data only, for example, if you were measuring temperature and water depth, each temperature measurement would belong with only one specific depth measurement (both taken atthe same place). If you mixed the matched pairs up the data would be meaningless.Examples of where you might use Spearmans rank correlation:à ¯Ã‚ ¿Ã‚ ½Is there a correlation between temperature and height up a mountain?à ¯Ã‚ ¿Ã‚ ½Is there a correlation between mouse density and proximity to a cheese factory?à ¯Ã‚ ¿Ã‚ ½Is there a correlation between current speed and mayfly nymph abundance?à ¯Ã‚ ¿Ã‚ ½Is there a correlation between cigarette smoking and low intelligence?à ¯Ã‚ ¿Ã‚ ½Is there a correlation between species diversity and height on the seashore?Using Spearmans rank correlationUse a Spearmans rank correlation test when youve got two variables and you want to see if they are correlated. Your calculated Spearmans rank correlation coefficient (rs) lets you test to see if youve got a correlation between two variables, i.e. if a change in one variable is accompanied by a change in the other variable. It also measures the strengthof any correlation. You need at least eight pairs of data in matched pairs. Being inmatched pairs means that one piece of data is associated with only one other piece of data. For example, if you were measuring speed of hair growth at different ages, each hair growth measurement would belong with only one age measurement. If you mixed the matched pairs up the data would be meaningless.Types of correlationPositive correlationsFigure 1 shows the (fictional) relationship between amount of beer drunk and temperature. It seems that the hotter it gets, the more beer isdrunk. As one variable goes up so does the other . This is an example of aperfect positive correlation. If you calculated rs for these data you wouldget a value of _1 (plus one). Rarely will you get exactly _1 but stronglypositively correlated variables will usually give you a value approaching_1.Negative correlationsFigure 2 shows students happiness quotient plotted against the number ofhours spent doing statistics. Extraordinary as it may seem, it wouldappear that the longer you spend doing statistics the more unhappy youbecome. As one variable (time spent doing stats) goes up, the other(happiness quotient) goes down. This isa perfect negative correlation. If you calculated rs for these data youwould get a value of -1 (minus one). Rarely will you get exactly -1. Stronglynegatively correlated variables will usually give you a value approachingConclusion-I found out that overall as the temperature increased the colour released was darker, i.e. more pigmentation was released. This agrees with my prediction and shows that at lower t emperatures the colour did not leak as much but at higher temperatures the cell loses its permeability. This is because by increasing the temperature the structure of the membrane changes as the proteins loses their tertiary structure and begins to denature. This is because the interactions of the bonds that hold the protein in its structure are weak and easily altered by physical changes. This tertiary structure will begin to breakdown the proteins and lose the ability to control what passes through and so more betalins will pass through and so there will be a greater pigment loss. As a result the lipid biayer may also be begin to break and larger gaps start to form between the phospholipids molecules and more pigments (betalins) can move into and out of the cell.The line of best fit on the graph is shows that the temperature is proportional to the light absorbency. However it is not a strong positive correlation. For temperatures below 45C there was little difference between the t emperatures. However after 45C there is a huge difference between. The temperatures. I think this is because.The results in the graph indicate that overall as the temperature increases the permeability of the cell membrane decreases. The graph has produced a curve rather than the expected straight line. I think that this could have been because many of the experiments had to be reheated and so it affected the membrane.I feel that the graph can support a valid conclusion as it agrees with my prediction. The absorbency figure goes up steadily and this shows that there was a relationship between the temperature and the membrane permeability. I did not also have anomalies, which I think make the results more reliable.Although my results seem valid I acknowlede that they are not very relaible as I only have one set of resullts. Consequentrly I cannot check the precision of the results or the degree of accuracy I do not have other set of results to compare with. However the above results on their own do look precise with a positive correaltion.Although the results are good enough I understand that they are not completely accurate. Most importantly I know that because we did not have the availability of water baths it was very difficult to maintain the right temperature. Consequently although I heated the temperature slightly above what was needed to allow time for it to cool the temperature fell very quickly. As a result I had to (re)heat the solutions, including the ones with ice. Also as a result of this I may have moved some test-tubes more than others which could have affected the permeability of the membrane.Another error could have been my visibility. I understand that because I was wearing goggles it could have slightly obscured my view. Consequently when checking the temperature with the thermometer, whilst measuring the volume and when stopping the clock after 20 minutes I could have misread the measurements/temperature/time. Thus my reaction time when stop ping and even starting the clock each time could have been faster or slower giving unequal times. Another possibility could be that because I used the same thermometer in between the experiments it could have transferred some particles from one to another and so affect the reliability. Furthermore when placing the curvettes in the colorimeter the time it took to collect the solutions and place them in the slot could have varied, which in turn could have affected the results.Evaluation- Overall the procedure used was suitable, however to obtain more reliable and valid results changes/improvements could be made.If a trail run was carried out, it could have been used to recognise weaknesses in the procedure so that the actual experiment gave more reliable results and fewer errors.Each temperature was only carried out once, to get more reliable and accurate and valid results could have carried it out at least 1 more time, or get another students set of results and combine them to get an average. could have obtained an averageWhen using the cork borer to obtain the beetroot pieces the samples were not obtained from the same area. It is important to take the pieces from a similar region of the beetroot as each type of tissue will have a different water potential and so it is important to use the same.. This could affect the results as different parts of the beetroot could contain more betalin than others, which would mean that that the more diffusion would happen in the beetroot pieces which contained more betalin when heated. By making sure that each beetroot was cut out of the same place in the beetroot it would ensure a more accurate set of results as all the pieces would contain the same amount of dye.When using the cork borer although I used the same size ones, it is a possibility that I may not have got the same volume of shape each time. Also when measuring the 1cm pieces and cutting with a knife, there is bound to have been some even slight difference. As an alternative to using a ruler to measure the beetroot pieces I could have used another piece of equipment such as vernier clips to give far more accurate measurements. eyepiece graticule. For small items, a standard ruler will give anaccuracy to 1 mm; vernier callipers will give an accuracy to 0.1 mm. Larger objects or distances may require a tape measure, which will have an accuracy that depends on the scale.During the experiment I had goggles on which could have obscured my view. I understand they are needed for safety; nevertheless they could have affected my visibility when measuring the volumes and identifying the temperature as well as stopping the timing. Instead of relying on a stop clock, an automatic clock or timer could be used that would indicate when 20 minutes were over. My eyesight could have contributed to some inaccuracy. Because I did not use an automatic stop clock it could be possible that I stopped the stopclock late. This could mean that the beetroot pieces wer e in for longer than 30 minutes. To avoid this next time I should use an automatic stopclock.Also whilst handling the beetroot pieces I did not take great care to ensure that I handle them with care. It could be possible that when placing them in the tubes I just threw them in which could have caused damage to the membrane. This could possibly cause more of the dye to leak out.Also the experiment was only carried out on a small scale. To obtain better results I think the temperature gap between each tube should have been 10C so that the trend and point where the membrane denatures can be distinguished more easily. However I feel that my results show fairly well how a membrane functions under temperature change.The other difficultly was getting the right temperature. Because no water baths were available I used a kettle to heat the water. Water baths would have been much more reliable and sensible to use as the bath could heat each water to the required temperature as they are thermo statically controlled. The problem with using the kettle was that it can only heat water till boiling point and so that meant I had to add tap water or ice to get the required temperature. Also the kettle can contain many impurities which I was not aware of. When conducting this experiment next time I will make sure that if water baths are not available I will use a Bunsen burner to heat the water as this way I can get the required temperatures more easily.I realise that a systematic error in the procedure was that I used the same thermometer for each temperature. This could have transferred some particles. If available, water baths would be a better option to use.I could have used Electronic balancesI would also use a smaller range of temperatures, starting with 10 to about 50,Dropping pipettesPasteur pipettes (also called dropping pipettes) are designed to give a constant volume in each drop, although they are not very accurate. They should be held upright (rubber bulb at the top) during use. To get a drop to f

Free Essays on Ken Kesey Author Analysis

Ken Kesey was born in 1935 in La Junta, Colorado. â€Å"After being elected the boy most likely to succeed by his high school, Kesey enrolled in the University of Oregon. He won a fellowship to a creative writing program at Stanford University† (Leeds 3). While he was there, Kesey became a volunteer in a program to test the effects of new drugs at the local Veteran's Administration hospital. During this time, he discovered LSD and became interested in studying alternative methods of perception. He soon took a job in a mental institution, where he spoke extensively to the patients. He began writing One Flew Over the Cuckoo's Nest, which is based on some of his experiences with mental patients. In the conflict between Nurse Ratched and McMurphy, the novel dealt with individuality and rebellion against conformity. The novel was published in 1962 and was an immediate success. â€Å"With the money earned from the book, Kesey purchased a farm in California where he and his friends experimented heavily with LSD. He believed that using LSD to achieve altered states of mind could improve society† (Leeds 23). Kesey, a high profile sixties personality and up and coming author, took his beliefs about authority, government and society, and put it into his writing. One Flew Over The Cuckoo’s Nest embodies these beliefs and their influence can be strongly felt. In 1975, a movie version of Kesey's novel was released without his permission. â€Å"It was quite successful, but many people who had read the novel disapproved, saying that the movie did not accurately represent the events of the novel† (Leeds 40). â€Å"The book shows great illustration from Chief Bromden, better then in the movie† (Gatto 68). In the book Chief Bromden gives his interpretation of the Combine and how Nurse Ratched runs it. The movie shows many differences from the book. Like each character has their own attitude about the ward and the patients. Mrs. Ratched ... Free Essays on Ken Kesey Author Analysis Free Essays on Ken Kesey Author Analysis Ken Kesey was born in 1935 in La Junta, Colorado. â€Å"After being elected the boy most likely to succeed by his high school, Kesey enrolled in the University of Oregon. He won a fellowship to a creative writing program at Stanford University† (Leeds 3). While he was there, Kesey became a volunteer in a program to test the effects of new drugs at the local Veteran's Administration hospital. During this time, he discovered LSD and became interested in studying alternative methods of perception. He soon took a job in a mental institution, where he spoke extensively to the patients. He began writing One Flew Over the Cuckoo's Nest, which is based on some of his experiences with mental patients. In the conflict between Nurse Ratched and McMurphy, the novel dealt with individuality and rebellion against conformity. The novel was published in 1962 and was an immediate success. â€Å"With the money earned from the book, Kesey purchased a farm in California where he and his friends experimented heavily with LSD. He believed that using LSD to achieve altered states of mind could improve society† (Leeds 23). Kesey, a high profile sixties personality and up and coming author, took his beliefs about authority, government and society, and put it into his writing. One Flew Over The Cuckoo’s Nest embodies these beliefs and their influence can be strongly felt. In 1975, a movie version of Kesey's novel was released without his permission. â€Å"It was quite successful, but many people who had read the novel disapproved, saying that the movie did not accurately represent the events of the novel† (Leeds 40). â€Å"The book shows great illustration from Chief Bromden, better then in the movie† (Gatto 68). In the book Chief Bromden gives his interpretation of the Combine and how Nurse Ratched runs it. The movie shows many differences from the book. Like each character has their own attitude about the ward and the patients. Mrs. Ratched ...

Ethical issues in packaging practices Research Paper - 1

Ethical issues in packaging practices - Research Paper Example In accordance with the present trend of the global business environment, it is often observed that the organizations seek to invest on exceptional and creative packaging practices within the business process to draw a large number of potential users for their offerings. It has been observed from several decades that the global marketers are increasingly executing packaging practices to attract customers towards their range of offerings. Nevertheless, at times it is apparently recognized that the producers in the global business environment tend to mislead their consumers through practicing different types of conspicuous packaging of their products (Fan, 2005). Emphasizing upon the present environmental concern, the primary objective of this paper is to demonstrate an effective analysis on different ethical issues which are created by the modern business organizations in terms of utilizing their packaging practices. Moreover, the discussion of this paper will further focus on highligh ting major impacts of packaging practices and their potential adverse impacts on the environment. At the end of this paper, prospective suggestive measures will also be represented in order to prevent the impact of packaging practices on the global environment. II. Ethical Issues in Packaging Practices Marketing activities of the organizations are often recognized by the customers as one of the most unethical elements of the business strategies. This statement can be identified as one of the major drawbacks of organizational marketing activities especially in terms of packaging practices. In relation to the present rapidly increasing competitive business environment, organizations often tend to involve various unethical practices concerning their packaging and labeling of products (Bone & Corey, 2000). Different studies concerning the notion of packaging practices have depicted diverse types of ethical issues that the marketers raise in terms of achieving competitive position. In th is regard, it can be recognized from the study of Shimp (2003) that the notion of packaging significantly tends to involve four types of ethical issues. In this context, the marketers face the issues concerning the label information, safety, and label graphics along with environmental issues. II. a. Label Information The label information of the product packaging helps the customers to clearly identify the features, ingredients, price along with other important messages through which they can identify their exact expectations. Moreover, the labeling of products also ensures the customers in terms of availing appropriate products as per their needs and desires. However, marketers in the present business world are frequently involved on misleading their customers through presenting imaginary information concerning their product characteristics. For instance, the information concerning low fats/cholesterol or purity of contained ingredients within the products are frequently practiced by a few of the food processing organizations. In addition, the ethical issue relating to represent information differently in the similar category of products is also practiced by the marketers, which enables them to attract new customers segments for their products (Shimp, 2003). Therefore, it can be stated that

Strategy and strategic management Essay Example | Topics and Well Written Essays - 2750 words

Strategy and strategic management - Essay Example After developing plans, they provide resources that facilitate the implementation of policies and after a specified time, they evaluate whether the goals have been accomplished. Mission statements help managers to set the company direction. Various theories have been put forward by scholars regarding what strategic management entails. The most common is the ten schools of thought developed by Mintzberg. He classifies strategy into three categories namely; prescriptive, descriptive and configuration (Mintzberg, 1990). This theory helps to interpret the process of strategic planning and be able to plan even when there is change in environment as a result of internal or external factors. This paper is a critique of the concept of strategy, the strategy process and how it can be drawn upon and used by contemporary built environment organizations within current climate. Strategy is a plan of action directed towards achieving an organization’s goals and objectives. Managers set the organizational strategies and goals and also device ways of achieving them. Different managers use different approaches to strategize and Mintzberg categorizes them into ten schools for better understanding and thereafter classifies them into three broad categories depending on their purpose. They include; prescriptive, descriptive and configurative (Whittington, 2000). These involve designing, analysis and positioning for the future. The design school of thought is concerned with ways of developing strategies. The manager evaluates the strengths and weaknesses of the organization against the opportunities and threats which are external. Based on the result, the manager is able to formulate a strategy which addresses the shortcomings and tries to maintain a balance between the two. The organizational strategy does not have to be complicated and can be understood and implemented by all staff members while on the other hand, it is static and hence not suitable since

The History of Love by Nicole Krauss Essay Example | Topics and Well Written Essays - 750 words

The History of Love by Nicole Krauss - Essay Example The History of Love is a novel that revolves around the lives of three people. One of them is Leo Gursky, an ageing man living in perpetual fear of losing his life and feeling that this will happen unnoticed. He prepares for death in a shabby apartment in downtown Manhattan. The second is Leo, a Jew who made his way out of Poland as the Nazi advanced during the 2nd World War. When the author introduces Gursky, he seems to be weary of life after all that he has lost. While he lived in Poland, he was in love with a girl named Alma, to whom he had written a book and named all the female characters in the book after, a book also called â€Å"The History of Love†. However, Leo lost Alma when she moved to America and married someone else after she thought him dead in the Nazi Holocaust. Later on, when Leo moves to America, he leaves this novel to a friend who later tells him that he lost it. Although, Leo is able to survive the Nazi and their war and finally gets to the United State s, he does not have anything left. This is because he has lost the woman he loved and the book in which he chronicled all the memorable incidences that happened in his life. The novel arouses a feeling of sympathy for the losses that this kind man has experienced (Kuster 33). Alone in his house, in Manhattan, having lost the son that he loved so much, he is representative of what happens during old age, and what old people go through regarding their memories of loss. Leo finds it impossible to get over his love and loss of Alma and cannot move on with his life. The loss he felt when Alma left was a lot, however, he still loves her as much when she becomes an old woman, losing her life in a hospital as he did when she was eleven years of age. As she dies, Leo is again confronted by loss and he goes to sit by her side every day during after-hours while telling her jokes: â€Å"She was tiny and wrinkled and deaf as a door knob. There was so much I should have said†¦. yet I told h er jokes† (Krauss 65). When considering the theme of loss in The History of Love, it is impossible not to notice the way that the author uses language to chronicle the feelings of loss. "She was gone, and all that was left was the space you'd grown around her, like a tree that grows around a fence. For a long time, it remained hollow. Years, maybe, and when at last it was filled again, you knew that the new love you felt for a woman would have been impossible without Alma. If it weren't for her, there would never have been an empty space or the need to fill it" (Krauss 189). The passage in the novel is a stark description of the feeling of loss, as well as the feeling of void after as a distraught Leo Gursky experienced it. After Leo Gursky had settled, in America, he got himself a job as a locksmith at a shop owned and run by his cousin. As the years pass by, Leo Gursky grows old and begins to lose his health in terms of his heart becomes weaker. He becomes lonely and frighte ned in regards to his unfulfilled life and losses, which he suffers (Kuster 43). He watches on as the son he lost grows up and becomes a renowned writer before he loses him when he grows old and passes away. He has had to do all this from a distance since he lost his son’s love. He earlier stated that he had a son named Isaac who is not aware of his existence. As an old man, Leo attempts to do anything with the aim of making sure that when he dies; he is not forgotten, the memories others have of him are not lost and he does not die as an invisible man. He poses nude for various arts classes, hassles the deliverymen and spills milk at a Starbucks store. He does these to ensure that people remember him and his life does not get lost. He continues with his life, but does not know

Importance Of Self Reflection In Learning

Importance Of Self Reflection In Learning Abstract Self reflection is one of the important phases of learning. As a good designer, self reflection is essential in order to be a great graphic designer. The artwork that I had chosen is the school magazine cover design of SMJK Nan Hwa, Sitiawan that I had done during my internship at SIN BOON BENG PRINTING SDN BHD. SIN BOON BENG PRINTING SDN BHD is a printing company that provides a platform for people to have their own customization design for their own printing order especially the government education department like the primary schools, secondary schools, high schools, colleges and others. The theme of the school magazine cover for this year was to revise from the past and learn something new. The concept of the cover design is to design the cover that suits their theme. Besides, the client also wanted the oriental feel in the cover design mainly because of the identity of the school. In this essay, I will be evaluate and examine myself through this artwork. This essay will be focus ing on evaluating the influences on my design styles, design theories application, the design trends, appropriateness of the context, ethics and so on. Besides, I will also reflecting myself on the design works. I will explain the weaknesses and problems that I had faced in the design process. Each step of the design process will be explaining thoroughly in this essay. Introduction As a knowledgeable graphic design graduate without any personal reflection from time to time, is not compatible to be a great designer. Self reflection is a form of personal response to the experiences, situations, events or even the surroundings that happened around us. The reflective essay can be considered as a mirror that reflects our own personalities and the growth process in us. This is very crucial to everyone especially a designer like us. Reflective essay helps to find out our own personality, ability, design style and others. It can be considered as a processing phase where thinking and learning take place and it will help us to be a greater designer in the future. Therefore, the artwork chosen for this reflective essay is the school magazine cover design of SMJK Nan Hwa, Sitiawan for the year 2010. This artwork was produced during the fourteen weeks of internship at SIN BOON BENG PRINTING SDN BHD. As one of the member of SIN BOON BENG PRINTING SDN BHD, I was assigned to do the school magazine cover design for SMJK Nan Hwa, Sitiawan. This is one of the designs that I had given one hundred percent freedom in doing the design based on the clients requirements which means I did the design individually. Every time I start a design, I like to do some brainstorming and mind mapping for further idea generation. Since the school magazines theme is given by the school, I tried to find the denotative and connotative meaning of the theme. Besides, I also did some research on the layout design in order to do a better cover design for the school. I tried to refer to the resources of the company and also some previous design they had did for the school to gain some inspiration. Once I had done with the cover design, I showed it to my supervisor before it is shown to the client. I had done few designs for the school. The final artwork that had chosen is mainly because I was able to create the bond between the design and the theme. My supervisor and mentor had given me some comments for improvements during the design process. This assignment was actually quite challenging. This is because I need to think about the idea and concept all by myself. My supervisor and mentor just gave me some opinions and comments to improve my design. I was not given any guidance to do the design except the clients requirements. In this assignment, I had tried very hard to implement the knowledge that I had learnt in the university. After I did the design, the final artwork was email to the client and the client was quite satisfied about it. Scope of study This study focuses on evaluating and examines the reflecting process on my artwork. This report attempts to present a preview of developments and the involvement in the general area of design process development. Therefore it will address issues such as design methodologies, markets design trend, application of knowledge and others. The development process will be addressed through different features of case studies, design theories and methodologies in this essay. This study summarizes some of the information, as well as information from the previous artworks, in an attempt to illustrate the evolution of my design growing process. It reviews, as well, researches, knowledge and experiences that brought changes in my design career. Objectives/ Aims of essay Self reflection is a very important phase for everyone especially a young designer like me. It is highly beneficial to students development and provides a great value in students graduated life. Those students who are well equipped and managed to done well during this phase probably might have a great success in their career life. It also prepared us to face the real industry working environment and make us understand other strengths and weakness so that we can improve ourselves and prepared ourselves for a better future. Self reflective writing indirectly develops our critical thinking and making us to consider the comments or critics of our design so that we can improve about it. The objectives of self reflective essay are as followings: To provide us an opportunity to practice, evaluate and examine our knowledge in our learning process. Through self reflections, we are able to gain practical experience that is related to our course and yet enhance our basic skills that have been learnt in university through this self reflection session. Furthermore, through this self reflection session, we had a chance to implement what we have learned in the class and yet we are able to learn even more no matter in design sense or skills throughout this session. To equip ourselves. It is essential that every designer should cultivate self reflection in our design life so that our critical thinking, creativities and skills will be improved in the passage of time. Learning is a life-long process. Learning through our own mistakes and experiences even required more time and more difficulties because we need to evaluate ourselves and learn from our mistakes. It also helps to speed up the learning process of how our knowledge could be applied in the reality. To develop our critical thinking and enhance our creativities. Critical thinking is a mindset that is able to conceptualize, apply, analyze, synthesize, and/or evaluate information through observations, experiences, reflections, reasoning, or communications. Self reflections help to develop our critical thinking whereby we are able to process the information, comments or critics and accept it with ordinary mindset. We are able to accept every critic and listen to the comments around us. We are also able to analysis the comments and critics in order to improve ourselves for a better future. Rationale of essay The study of reflecting ourselves on the graphic design and multimedia field through previous artworks can be a learning paradigm in the higher level to enhance our knowledge and communication skills as well. This is not only basic education be learned by todays students but acquisition of learning skills and knowledge for gainful employment and full participation in countrys society. The essays goal is designed to help students improve academic competence, develop employability skills, implement a career plan and participate in a career pathway in preparation for post secondary education or careers in the graphic design and multimedia field after graduating. This goal can be achieved through the self reflection process that can guide and evaluate ourselves and try to learn from the mistakes. The output of this study is a source material that the teachers can assimilate and disseminate by diffusion and induction technique. Problem Statement In every design process, there are always many problems occurred during the design phase. I had several problems in designing the school magazine cover. The school had provided their own theme design in a circle shape and they requested to remain it. As the result, I had limited to do a circle design on the surrounding of the theme. As the logo design for the school theme is in white colour, I need to consider the suitable colours for the cover. Besides, the client requested to have the oriental feel on the cover and yet can bring up the theme. In order to solve the problems, I did a lot of research on the oriental at first. I also did the research on the theme of the school where it actually came from Confucius, to learn something new from the past.

Spoon River in History Essay -- essays research papers

With the coming of the new century America under goes a change led by many different events. The collection of poems written in Lee Masters book Spoon River Anthology portrays the typical small town at the end of the 19th century and the beginning of the 20th century. Show the different social, economical, and political trend and influences throughout the United States.   Ã‚  Ã‚  Ã‚  Ã‚  The city of Spoon River went through many of the same social trends that the United States experienced like social Darwinism and the change in woman roles in society. The idea of social Darwinism had its part in the country as well as in Spoon River. In Spoon River Anthology the character Felix Schmidt found out the hard way of the concept of social Darwinism where only the strong person wins or in this case the one with more money wins. Schroeder the fisherman also shows the example of the new concept spreading through the country. Schroeder tells of the conflict between many different types of animals in relation to the Felix Schmidt case how Schmidt was the weaker of the two and loses all his property including his little doll type house. Schmidt had to become a tenant farmer, since he became a tenant farmer he had to give a portion of his profits and income to Christian Dallman who was Felix’s landlord.   Ã‚  Ã‚  Ã‚  Ã‚  As Spoon River grew the social conflict grew between men and women in contrast to the role women had during the time period. The typical roles of men and women had a very strong line of distinction between them. However with time comes change these roles slowly began to intermix, mostly the role of women began to change. Women become more active in the community and the work place. With women becoming more involved in the jobs such as teachers it led to a very new and different life style. Teachers during this time were mostly men, however with the involvement of women and the racial movement which lead the 19th amendment giving women the right to vote and to end woman suffrage. During the many different wars that the United States got into the wounded was cared for by nurses, which were most of the time women. Women’s roles in the beginning of the 20th century had changed.   Ã‚  Ã‚  Ã‚  Ã‚  As the economical ups and down took its tolls on the United States it also hit Spoon River hard. With the country going in and... ...talk with the most powerful man in the United States government shows that United States wanted to be more into the government policy. With many new presidents moving into office each had a different idea on how to help the United States. McKinley thought that if he would make a tariff that would raise the price on manufactured goods by 48% that it would scare off the people from buying products that were not American made, which would help raise the economy. What it did was just make things worse because other countries were putting high tariffs on American products so it hurt the United States businesses both large and small companies. . The people of Spoon River had encountered many different trends that had shaped the community into a typical town in the Unites States. From social Darwinism to the depression of 1893 to many different ways the railroad influenced the people and society of Spoon River. As Spoon River grows and ages with the country there will be many more and different trends and influences on the thriving little town. With many things to come from all of the different social, economical and political fields in the Unites States.   Ã‚  Ã‚  Ã‚  Ã‚